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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
15/03/1999 |
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Data da última atualização: |
15/03/1999 |
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Autoria: |
ARAUJO, J. L. S.; RUMJANEK, Norma G.; MARGIS-PINHEIRO, M. |
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Título: |
Expression of heat shock proteins in bean plants (Phaseolus vulgaris L.) submitted to high temperatures. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 26., maio 1997, Caxambu. Resumos... Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1997. p.34. |
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Idioma: |
Inglês |
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Conteúdo: |
Leguminous plants interact with rhizobia to form special structures (nodules) where N2 fixation occurs. High temperature stress is a major factor limiting nitrogen fixation in the tropical areas. Although several heat-tolerant Rhizobium strains capable of nodulating bean have been isolated, common bean plants are considered to be sensitive. Consequently, the selection of plant varieties adapted to high temperatures constitutes an important strategy to improve nitrogen fixation in tropical areas. When subjected to high temperatures, a wide variety of species induce synthesis of a set of proteins known as heat shock proteins (HSP). Several studies have demonstrated that HSPs are related to the acquisition of thermal tolerance. In plants, the low molecular weight HSPs constitute the most abundant and diverse group of proteins synthesized in response to heat stress. The aim of this study was investigate the expression pattern in different varieties of bean plants submitted to high temperatures. We started this analysis by determining the conditions for mRNA HSP induction in common bean. Cloned cDNAs of wheat (WHSP 16,9; class I) and of Zea mays (ZMHSP 27; class II) were used as probes. Bean plants (cv IPA 7) growing at 28 graus C were submitted to several heat shock conditions and the analysis of HSP expression were performed using northern-blots. Cowpea and maize were included as controls. The monocotyledon probes used were capable of hydridizing under several heat shock conditions, but at different levels... MenosLeguminous plants interact with rhizobia to form special structures (nodules) where N2 fixation occurs. High temperature stress is a major factor limiting nitrogen fixation in the tropical areas. Although several heat-tolerant Rhizobium strains capable of nodulating bean have been isolated, common bean plants are considered to be sensitive. Consequently, the selection of plant varieties adapted to high temperatures constitutes an important strategy to improve nitrogen fixation in tropical areas. When subjected to high temperatures, a wide variety of species induce synthesis of a set of proteins known as heat shock proteins (HSP). Several studies have demonstrated that HSPs are related to the acquisition of thermal tolerance. In plants, the low molecular weight HSPs constitute the most abundant and diverse group of proteins synthesized in response to heat stress. The aim of this study was investigate the expression pattern in different varieties of bean plants submitted to high temperatures. We started this analysis by determining the conditions for mRNA HSP induction in common bean. Cloned cDNAs of wheat (WHSP 16,9; class I) and of Zea mays (ZMHSP 27; class II) were used as probes. Bean plants (cv IPA 7) growing at 28 graus C were submitted to several heat shock conditions and the analysis of HSP expression were performed using northern-blots. Cowpea and maize were included as controls. The monocotyledon probes used were capable of hydridizing under several heat shock c... Mostrar Tudo |
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Palavras-Chave: |
BNF; FBN; Fixacao biologica de nitrogenio; Nitrogen fixing bacteria. |
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Thesagro: |
Calor; Feijão; Nódulo; Phaseolus Vulgaris; Proteína; Rhizobium; Stress; Temperatura. |
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Thesaurus Nal: |
heat stress; kidney beans; proteins; root nodules. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02577naa a2200337 a 4500
001 1620981
005 1999-03-15
008 1997 bl uuuu u00u1 u #d
100 1 $aARAUJO, J. L. S.
245 $aExpression of heat shock proteins in bean plants (Phaseolus vulgaris L.) submitted to high temperatures.
260 $c1997
520 $aLeguminous plants interact with rhizobia to form special structures (nodules) where N2 fixation occurs. High temperature stress is a major factor limiting nitrogen fixation in the tropical areas. Although several heat-tolerant Rhizobium strains capable of nodulating bean have been isolated, common bean plants are considered to be sensitive. Consequently, the selection of plant varieties adapted to high temperatures constitutes an important strategy to improve nitrogen fixation in tropical areas. When subjected to high temperatures, a wide variety of species induce synthesis of a set of proteins known as heat shock proteins (HSP). Several studies have demonstrated that HSPs are related to the acquisition of thermal tolerance. In plants, the low molecular weight HSPs constitute the most abundant and diverse group of proteins synthesized in response to heat stress. The aim of this study was investigate the expression pattern in different varieties of bean plants submitted to high temperatures. We started this analysis by determining the conditions for mRNA HSP induction in common bean. Cloned cDNAs of wheat (WHSP 16,9; class I) and of Zea mays (ZMHSP 27; class II) were used as probes. Bean plants (cv IPA 7) growing at 28 graus C were submitted to several heat shock conditions and the analysis of HSP expression were performed using northern-blots. Cowpea and maize were included as controls. The monocotyledon probes used were capable of hydridizing under several heat shock conditions, but at different levels...
650 $aheat stress
650 $akidney beans
650 $aproteins
650 $aroot nodules
650 $aCalor
650 $aFeijão
650 $aNódulo
650 $aPhaseolus Vulgaris
650 $aProteína
650 $aRhizobium
650 $aStress
650 $aTemperatura
653 $aBNF
653 $aFBN
653 $aFixacao biologica de nitrogenio
653 $aNitrogen fixing bacteria
700 1 $aRUMJANEK, Norma G.
700 1 $aMARGIS-PINHEIRO, M.
773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 26., maio 1997, Caxambu. Resumos... Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1997. p.34.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
14/10/2020 |
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Data da última atualização: |
16/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PINHEIRO-LIMA, B.; PEREIRA-CARVALHO, R. C.; ALVES-FREITAS, D. M. T.; KITAJIMA, E. W.; VIDAL, A. H.; LACORTE, C.; GODINHO, M. T.; FONTENELE, R. S.; FARIA, J. C.; ABREU, E. F. M.; VARSANI, A.; RIBEIRO, S. G.; MELO, F. L. |
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Afiliação: |
BRUNA PINHEIRO-LIMA, UNB; RITA C. PEREIRA-CARVALHO, UNB; DIONE M. T. ALVES-FREITAS; ELLIOT W. KITAJIMA, USP; ANDREZA H. VIDAL, UNB; CRISTIANO CASTRO LACORTE, Cenargen; MARCIO T. GODINHO; RAFAELA S. FONTENELE, ARIZONA STATE UNIVERSITY, USA; JOSIAS CORREA DE FARIA, CNPAF; EMANUEL FELIPE MEDEIROS ABREU, Cenargen; ARVIND VARSANI, ARIZONA STATE UNIVERSITY, USA; SIMONE DA GRACA RIBEIRO, Cenargen; FERNANDO L. MELO, UNB. |
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Título: |
Transmission of the bean-associated Cytorhabdovirus by the whitefly Bemisia tabaci MEAM1. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Viruses, v. 12, n. 9, 1028, 2020. |
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ISSN: |
1999-4915 |
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DOI: |
https://doi.org/10.3390/v12091028 |
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Idioma: |
Inglês |
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Conteúdo: |
The knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown.This information is crucial for the understanding of virus?plant interactions, control strategies,and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plantsand are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers,and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papayacytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil,(ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here,we examine the potential of theBemisia tabaciMiddle East-Asia Minor 1 (MEAM1) to transmit BaCV,its morphological and cytopathological characteristics, and assess the incidence of BaCV across beanproducing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimentalconditions, byB. tabaciMEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean.Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viralparticles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests,identification of the induced symptoms, and the transmission assay. BaCV was detected in five out ofthe seven states in Brazil included in our study, suggesting that it is widely distributed throughoutbean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus. MenosThe knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown.This information is crucial for the understanding of virus?plant interactions, control strategies,and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plantsand are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers,and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papayacytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil,(ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here,we examine the potential of theBemisia tabaciMiddle East-Asia Minor 1 (MEAM1) to transmit BaCV,its morphological and cytopathological characteristics, and assess the incidence of BaCV across beanproducing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimentalconditions, byB. tabaciMEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean.Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viralparticles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests,identification of the induced symptoms, and the transmission assay. BaCV was detected in five out ofthe seven states in Brazil included in our study, suggesting t... Mostrar Tudo |
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Palavras-Chave: |
Common bean; Vector; Virus evolution; Whitefly. |
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Thesagro: |
Bemisia Tabaci; Feijão; Mosca Branca; Phaseolus Vulgaris; Vetor; Vírus. |
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Thesaurus NAL: |
Cytorhabdovirus; Virus transmission. |
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Categoria do assunto: |
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H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216635/1/viruses-12-01028.pdf
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Marc: |
LEADER 02812naa a2200433 a 4500
001 2125504
005 2020-10-16
008 2020 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $ahttps://doi.org/10.3390/v12091028$2DOI
100 1 $aPINHEIRO-LIMA, B.
245 $aTransmission of the bean-associated Cytorhabdovirus by the whitefly Bemisia tabaci MEAM1.$h[electronic resource]
260 $c2020
520 $aThe knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown.This information is crucial for the understanding of virus?plant interactions, control strategies,and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plantsand are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers,and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papayacytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil,(ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here,we examine the potential of theBemisia tabaciMiddle East-Asia Minor 1 (MEAM1) to transmit BaCV,its morphological and cytopathological characteristics, and assess the incidence of BaCV across beanproducing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimentalconditions, byB. tabaciMEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean.Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viralparticles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests,identification of the induced symptoms, and the transmission assay. BaCV was detected in five out ofthe seven states in Brazil included in our study, suggesting that it is widely distributed throughoutbean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus.
650 $aCytorhabdovirus
650 $aVirus transmission
650 $aBemisia Tabaci
650 $aFeijão
650 $aMosca Branca
650 $aPhaseolus Vulgaris
650 $aVetor
650 $aVírus
653 $aCommon bean
653 $aVector
653 $aVirus evolution
653 $aWhitefly
700 1 $aPEREIRA-CARVALHO, R. C.
700 1 $aALVES-FREITAS, D. M. T.
700 1 $aKITAJIMA, E. W.
700 1 $aVIDAL, A. H.
700 1 $aLACORTE, C.
700 1 $aGODINHO, M. T.
700 1 $aFONTENELE, R. S.
700 1 $aFARIA, J. C.
700 1 $aABREU, E. F. M.
700 1 $aVARSANI, A.
700 1 $aRIBEIRO, S. G.
700 1 $aMELO, F. L.
773 $tViruses$gv. 12, n. 9, 1028, 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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